IL-12 Antibody
IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN) -gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
P29459
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 168-195 from human IL12A was used as the immunogen for this IL-12 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, WB, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the IL-12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This IL-12 antibody is available for research use only.
Storage Conditions
Aliquot the IL-12 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the IL-12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, secreted
Image Legend
Western blot analysis of IL-12 antibody and MDA-MB231 lysate. Predicted molecular weight ~25 kDa (unmodified), ~35 kDa (glycosylated) .
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