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RAB7 Antibody

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

P51149

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 176-204 from human RAB7A was used as the immunogen for this RAB7 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the RAB7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This RAB7 antibody is available for research use only.

Storage Conditions

Aliquot the RAB7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the RAB7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Location

Cytoplasmic

Image Legend

RAB7 antibody flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control (top histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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