MICA Antibody

MICA is the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow cytometry: 1:25, Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25

UniProt

Q29983

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 68-97 from the human protein was used as the immunogen for this MICA antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, IF, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the MICA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MICA antibody is available for research use only.

Storage Conditions

Aliquot the MICA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the MICA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of MICA antibody at 1:2000 dilution. Lane 1: Jurkat lysate; 2: Daudi ; 3: A431; 4: MCF-7; 5: U-87MG; 6: THP-1; Predicted molecular weight: ~43 kDa but may be observed at 38-62 kDa depending on truncation and glycosylation level.