Retinoblastoma Antibody
RB1/Retinoblastoma is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
P06400
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 858-886 from the human protein was used as the immunogen for this Retinoblastoma antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, FACS, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Retinoblastoma antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Retinoblastoma antibody is available for research use only.
Storage Conditions
Aliquot the Retinoblastoma antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Retinoblastoma antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Rat
Image Legend
Western blot analysis of Retinoblastoma antibody and 293 lysate
Frequently Asked Questions
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