Fas Antibody / CD95
Fas is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:10-1:50
UniProt
P25445
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 185-211 from the human protein was used as the immunogen for this Fas antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the FAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Fas antibody is available for research use only.
Storage Conditions
Aliquot the Fas antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Fas antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membranous
Image Legend
Western blot analysis of Fas antibody and T47D lysate. Predicted molecular weight: ~38 kDa (unmodified), 40-55 kDa (glycosylated) .
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