CYP1A1 Antibody

CYP1A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. CYP1A1 gene has been associated with lung cancer risk.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50, IHC (Paraffin) : 1:10-1:50

UniProt

P04798

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 252-280 from the human protein was used as the immunogen for this CYP1A1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CYP1A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CYP1A1 antibody is available for research use only.

Storage Conditions

Aliquot the CYP1A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CYP1A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membrane

Image Legend

Confocal immunofluorescent analysis of CYP1A1 antibody with MDA-MB231 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .