PLK1 Antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100
UniProt
P53350
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 573-603 from the human protein was used as the immunogen for this PLK1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the PLK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PLK1 antibody is available for research use only.
Storage Conditions
Aliquot the PLK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the PLK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse
Image Legend
Western blot analysis of PLK antibody and A375 cell lysate.
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