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PLK1 Antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

P53350

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 573-603 from the human protein was used as the immunogen for this PLK1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PLK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PLK1 antibody is available for research use only.

Storage Conditions

Aliquot the PLK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PLK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Image Legend

Western blot analysis of PLK antibody and A375 cell lysate.

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