PAK6 Antibody

The PAK6 protein shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. PAK6 was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. The p21-activated protein kinase 6 gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

Q9NQU5

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 116-146 from the human protein was used as the immunogen for this PAK6 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PAK6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PAK6 antibody is available for research use only.

Storage Conditions

Aliquot the PAK6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PAK6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of PAK6 antibody in lysate from transiently transfected COS7 cells. Lane 1: negative control, 2: PAK1, 3: PAK2, 4: PAK4, 5: PAK5, and 6: PAK6-expressing cells.