PAK1 Antibody
PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:10-1:50, IHC (Paraffin) : 1:10-1:50
UniProt
Q13153
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 401-430 from the human protein was used as the immunogen for this PAK1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the PAK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PAK1 antibody is available for research use only.
Storage Conditions
Aliquot the PAK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the PAK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat, Bovine, Rabbit, Xenopus
Image Legend
Frequently Asked Questions
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