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MST1 Antibody (Mammalian STE20-like protein kinase 1)

MST1 is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it is possible that this protein induces the chromatin condensation observed in this process.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

Q13043

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 385-415 from the human protein was used as the immunogen for this MST1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the MST1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MST1 antibody is available for research use only.

Storage Conditions

Aliquot the MST1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the MST1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine

Image Legend

Western blot analysis of MST1 antibody and HL-60 cell lysate. Predicted molecular weight ~ 56 kDa.

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