MST1 Antibody (Mammalian STE20-like protein kinase 1)
MST1 is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it is possible that this protein induces the chromatin condensation observed in this process.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100
UniProt
Q13043
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 385-415 from the human protein was used as the immunogen for this MST1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the MST1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This MST1 antibody is available for research use only.
Storage Conditions
Aliquot the MST1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the MST1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine
Image Legend
Western blot analysis of MST1 antibody and HL-60 cell lysate. Predicted molecular weight ~ 56 kDa.
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