GAPDH Antibody

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

P04406

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 233-259 from the human protein was used as the immunogen for this GAPDH antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GAPDH antibody is available for research use only.

Storage Conditions

Aliquot the GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Chicken, Mouse, Pig, Rat

Image Legend

Western blot analysis of lysate from HeLa, HUVEC cell line (left to right) using GAPDH antibody; Ab was diluted at 1:1000 for each lane. Predicted molecular weight ~36kDa.