Anti-GAPDH Antibody
GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
P04406
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 62-91 from the human protein was used as the immunogen for this anti-GAPDH antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the anti-GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-GAPDH antibody is available for research use only.
Storage Conditions
Aliquot the anti-GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Image Legend
Western blot analysis of lysate from A375 cell line using anti-GAPDH antibody diluted at 1:500. Predicted molecular weight ~36kDa.
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