Anti-GAPDH Antibody

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

P04406

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 62-91 from the human protein was used as the immunogen for this anti-GAPDH antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the anti-GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This anti-GAPDH antibody is available for research use only.

Storage Conditions

Aliquot the anti-GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the anti-GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.