Acetylcholinesterase Antibody / ACHE

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunohistochemistry (FFPE) : 1:10-1:50, Flow cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

P22303

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 147-175 from the human protein was used as the immunogen for this Acetylcholinesterase antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, FACS, IF, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Acetylcholinesterase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Acetylcholinesterase antibody is available for research use only.

Storage Conditions

Aliquot the Acetylcholinesterase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Acetylcholinesterase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Guinea pig

Location

Cytoplasmic, membranous

Image Legend

Confocal immunofluorescent analysis of Acetylcholinesterase antibody with human NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . Actin filaments labeled with Alexa Fluor 555 Phalloidin (red) . DAPI used as a nuclear counterstain (blue) .