BRAF Antibody
BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL) . This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Flow Cytometry: 1:10-1:50
UniProt
P15056
Host
Rabbit
Reactivity
Human
Immunogen
This BRAF antibody was produced from rabbits immunized with B-RAF recombinant protein.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This BRAF antibody is available for research use only.
Storage Conditions
Aliquot the BRAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
BRAF antibody western blot analysis in (1) HeLa and (2) T47D lysate. Predicted size 85-95 kDa
Documents
Frequently Asked Questions
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