BRAF Antibody
BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL) . This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500, IHC (Paraffin) : 1:10-1:50
UniProt
P15056
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 580-609 from the human protein was used as the immunogen for this BRAF antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This BRAF antibody is available for research use only.
Storage Conditions
Aliquot the BRAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Chicken, Mouse
Image Legend
Western blot analysis of lysate from MCF-7 cell line using BRAF antibody. Predicted size 85-95 kDa
Frequently Asked Questions
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