B-RAF Antibody

B-RAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL) . This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

UniProt

P15056

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 379-408 from the human protein was used as the immunogen for this B-RAF antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the B-RAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This B-RAF antibody is available for research use only.

Storage Conditions

Aliquot the B-RAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the B-RAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Image Legend

Western blot analysis B-RAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (2) . Predicted size 85-95 kDa