B-RAF Antibody

BRAF is a human gene, with the protein more formally known as serine/threonine-protein kinase B-Raf. The protein is involved in sending signals inside cells, which are involved in directing cell growth. Drugs that treat cancers driven by BRAF mutations have been developed. Two of these drugs, Vemurafenib and Dabrafenib are approved by FDA for treatment of late-stage melanoma. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P15056

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 424-453 from the human protein was used as the immunogen for this B-RAF antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC, IF, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the B-RAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This B-RAF antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the B-RAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the B-RAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.