HIF1A Antibody
HIF1A functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
Q16665
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 519-547 from the human protein was used as the immunogen for this HIF1A antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, IF, FACS, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HIF1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HIF1A antibody is available for research use only.
Storage Conditions
Aliquot the HIF1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HIF1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Rat, Bovine
Location
Nuclear, possible cytoplasmic
Image Legend
Western blot analysis of HIF1A antibody and mouse NIH3T3 lysate. Routinely observed molecular weight: 100~120 kDa.
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Bovine Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit
Bovine Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit
