HIF-1 alpha Antibody
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:10-1:50, IHC (Paraffin) : 1:10-1:50
UniProt
Q16665
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 728-757 from the human protein was used as the immunogen for this HIF-1 alpha antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, WB, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the HIF-1 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HIF-1 alpha antibody is available for research use only.
Storage Conditions
Aliquot the HIF-1 alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear, possible cytoplasmic
Image Legend
Western blot analysis of HIF-1 alpha antibody and MDA-MB231 lysate. Routinely observed molecular weight: 100~120 kDa.
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