SMAD4 Antibody
In muscle physiology, SMAD4 plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP) -mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity) . [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
Q13485
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 400-428 from the human protein was used as the immunogen for this SMAD4 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, IF, FACS, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SMAD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SMAD4 antibody is available for research use only.
Storage Conditions
Aliquot the SMAD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat, Bovine, Pig
Image Legend
Fluorescent confocal image of HeLa cell stained with SMAD4 antibody at 1:25. SMAD4 immunoreactivity is localized to the cytoplasm and nucleus.
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