SMAD4 Antibody

SMAD4 is the common SMAD (co-SMAD) mediator of signal transduction by TGF-beta (transforming growth factor) . It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50

UniProt

Q13485

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 255-284 from the human protein was used as the immunogen for this SMAD4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SMAD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SMAD4 antibody is available for research use only.

Storage Conditions

Aliquot the SMAD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat

Image Legend

Confocal immunofluorescent analysis of SMAD4 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red) . DAPI was used as a nuclear counterstain (blue) .