C-Src Antibody
The gene for Src is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. It is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in Src could be involved in the malignant progression of colon cancer.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50
UniProt
P12931
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for this c-Src antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, IF, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the c-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This c-Src antibody is available for research use only.
Storage Conditions
Aliquot the c-Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the c-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from (1) human MCF-7, (2) rat PC-12, and (3) mouse C2C12 cell line using c-Src antibody at 1:1000.
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