LCK Antibody
LCK, which belongs to the SRC subfamily of Tyr protein kinases, may participate in antigen-induced T-cell activation. It binds to phosphatidylinositol 3'-kinase (PI3K) from T lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. LCK is bound to the cytoplasmic domain of either CD4 or CD8. This protein is involved in leukemias by a chromosomal translocation t (1;7) (p34; q34) which involves LCK and T-cell receptor beta chain (TCRB) genes.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
P06239
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A portion of amino acids 23-52 from the human protein was used as the immunogen for this LCK antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, IF, FACS, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the LCK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This LCK antibody is available for research use only.
Storage Conditions
Aliquot the LCK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the LCK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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