ACK1 Antibody / Activated CDC42 kinase 1
Activated CDC42 kinase 1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP) -stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1000, IHC (Paraffin) : 1:50-1:100
UniProt
Q07912
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 934-964 from the human protein was used as the immunogen for this ACK1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ACK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ACK1 antibody is available for research use only.
Storage Conditions
Aliquot the ACK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the ACK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine
Location
Cytoplasmic
Image Legend
Western blot testing of human brain lysate with ACK1 antibody at 1:1000. Predicted molecular weight ~114 kDa.
Documents
Frequently Asked Questions
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