PDGFR Antibody

As a receptor for PDGFA, PDGFR alpha promotes or inhibits cell proliferation and cell migration. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

UniProt

P16234

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 698-727 from the human alpha protein was used as the immunogen for this PDGFR antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PDGFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PDGFR antibody is available for research use only.

Storage Conditions

Aliquot the PDGFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PDGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat, Chicken, Xenopus

Location

Cytoplasmic, membrane

Image Legend

Western blot analysis of PDGFR antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRA gene (2) . Predicted molecular weight: 120-195 kDa.