ILK Antibody
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK is a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction.
Product Specifications
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:50-1:100, Western blot: 1:1000
UniProt
Q13418
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 225-253 from the human protein was used as the immunogen for this ILK antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ILK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ILK antibody is available for research use only.
Storage Conditions
Aliquot the ILK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the ILK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Mouse, Rat
Location
Cytoplasmic
Image Legend
Western blot analysis of ILK antibody and HeLa lysate. Expected molecular weight: 51-59 kDa.
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