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ILK Antibody

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK is a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction.

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (Paraffin) : 1:50-1:100, Western blot: 1:1000

UniProt

Q13418

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 225-253 from the human protein was used as the immunogen for this ILK antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IHC, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ILK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ILK antibody is available for research use only.

Storage Conditions

Aliquot the ILK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/ilk-antibody-f50638

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the ILK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Mouse, Rat

Location

Cytoplasmic

Image Legend

Western blot analysis of ILK antibody and HeLa lysate. Expected molecular weight: 51-59 kDa.

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