FGFR2 Antibody

Fibroblast growth factor receptor 2 is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

P21802

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 794-821 from the human protein was used as the immunogen for this FGFR2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the FGFR2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This FGFR2 antibody is available for research use only.

Storage Conditions

Aliquot the FGFR2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the FGFR2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Image Legend

Confocal immunofluorescent analysis of FGFR2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .