ALK Antibody

Anaplastic lymphoma kinase, a member of the insulin receptor subfamily of Tyr protein kinases, is an orphan receptor. It appears to play an important role in the normal development and function of the nervous system. This Type I membrane protein is expressed in brain and CNS and in the small intestine and testis, but not in normal lymphoid cells. A form of non-Hodgkin's lymphoma is characterized by a chromosomal translocation t (2;5) (p23; q35) that involves NPM1 and ALK. The protein contains 1 LDL-receptor class A domain and 2 putative MAM domains.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

UniProt

Q9UM73

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 636-666 from the human protein was used as the immunogen for this ALK antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ALK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ALK antibody is available for research use only.

Storage Conditions

Aliquot the ALK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the ALK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of ALK antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2) . Predicted molecular weight: 190-220 kDa.