E2F1 Antibody
E2F1 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
Q01094
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 342-371 from the human protein was used as the immunogen for this E2F1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the E2F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This E2F1 antibody is available for research use only.
Storage Conditions
Aliquot the E2F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the E2F1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of E2F1 antibody at 1:1000 dilution. Lane 1: NIH3T3 lysate; 2: A431; Predicted molecular weight: 48-60 kDa.
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