CDK4 Antibody
CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 (INK4a) . This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb) . Mutations in this gene as well as in its related proteins including D-type cyclins, p16 (INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of the gene have been reported.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:10-1:50, Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
P11802
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 273-305 from the human protein was used as the immunogen for this CDK4 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, IHC, WB, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the CDK4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CDK4 antibody is available for research use only.
Storage Conditions
Aliquot the CDK4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the CDK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear and cytoplasmic
Image Legend
Western blot analysis of CDK4 antibody and HL-60 cell lysate. Predicted molecular weight ~34 kDa.
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