CDK2 Antibody
CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) . Its activity is also regulated by its protein phosphorylation.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50
UniProt
P24941
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for this CDK2 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the CDK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CDK2 antibody is available for research use only.
Storage Conditions
Aliquot the CDK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the CDK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat, Bovine, Hamster, Xenopus
Image Legend
Cdk2 antibody western blot analysis in 293, HeLa lysate. Predicted molecular weight ~33 kDa.
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