IDH1 Antibody
IDH1 belongs to two distinct subclasses. The protein is the NADP (+) -dependent Isocitrate Dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [Wiki]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:10-1:50, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
O75874
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 116-143 from the human protein was used as the immunogen for this IDH1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, IF, WB, FACS, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the IDH1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This IDH1 antibody is available for research use only.
Storage Conditions
Aliquot the IDH1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/idh1-antibody-f50422
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the IDH1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Rat
Location
Cytoplasmic, nuclear
Image Legend
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Rabbit Monoclonal Cadherin-12 Antibody (403) [Alexa Fluor 532]