IDH1 Antibody

IDH1 belongs to two distinct subclasses. The protein is the NADP (+) -dependent Isocitrate Dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

O75874

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 116-143 from the human protein was used as the immunogen for this IDH1 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, IF, WB, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the IDH1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IDH1 antibody is available for research use only.

Storage Conditions

Aliquot the IDH1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the IDH1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Rat

Location

Cytoplasmic, nuclear

Image Legend

Western blot analysis of lysate from (1) HepG2, (2) MCF-7 cell line, (3) human liver and (4) rat liver tissue using IDH1 antibody at 1:1000. Predicted molecular weight ~46 kDa.