IDH Antibody

The protein is the NADP (+) -dependent Isocitrate Dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

O75874

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 63-90 from the human protein was used as the immunogen for this IDH antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, FACS, IF, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the IDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IDH antibody is available for research use only.

Storage Conditions

Aliquot the IDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the IDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Rat

Location

Cytoplasmic, nuclear

Image Legend

Confocal immunofluorescent analysis of IDH antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI nuclear counterstain (blue) .