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FASN Antibody

FASN (Fatty acid synthase) is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:200, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P49327

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 942-973 from human Fatty acid synthase was used as the immunogen for this FASN antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, WB, IHC, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the FASN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This FASN antibody is available for research use only.

Storage Conditions

Aliquot the FASN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the FASN antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot analysis of FASN antibody in Ramos lysate. Predicted molecular weight ~ 270 kDa.

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