DHFR Antibody

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

P00375

Host

Rabbit

Reactivity

Mouse

Immunogen

A portion of amino acids 135-164 from mouse Dihydrofolate reductase was used as the immunogen for this DHFR antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the DHFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This DHFR antibody is available for research use only.

Storage Conditions

Aliquot the DHFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of DHFR antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the DHFR gene. Predicted molecular weight ~22 kDa.