Cytokeratin-18 Antibody

KRT18 is the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament family. They are expressed in single layer epithelial tissues of the body. Mutations in its gene have been linked to cryptogenic cirrhosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50, IHC (Paraffin) : 1:10-1:50

UniProt

P05783

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 401-430 from the human protein was used as the immunogen for this Cytokeratin-18 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IF, IHC, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Cytokeratin-18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Cytokeratin-18 antibody is available for research use only.

Storage Conditions

Aliquot the Cytokeratin-18 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Cytokeratin-18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Confocal immunofluorescent analysis of Cytokeratin-18 antibody with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .