SMAD2 Antibody
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:100, Western blot: 1:1000, Flow Cytometry: 1:10-1:50
UniProt
Q15796
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 97-125 from the human protein was used as the immunogen for this SMAD2 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, WB, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SMAD2 antibody is available for research use only.
Storage Conditions
Aliquot the SMAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Rat, Bovine, Pig, Chicken, Zebrafish, Drosophila
Image Legend
Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody at 1:100. Note the highly specific localization of the SMAD2 to the nucleus.
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