Aurora B Antibody

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. STK12 (Aurora kinase B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

Q96GD4

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 283-313 from the human protein was used as the immunogen for this Aurora B antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Aurora B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Aurora B antibody is available for research use only.

Storage Conditions

Aliquot the Aurora B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Aurora B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of Aurora B antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AURKB gene (2) .