LKB1 Antibody

LKB1 is a tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

Q15831

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 19-48 from the human protein was used as the immunogen for this LKB1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the LKB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This LKB1 antibody is available for research use only.

Storage Conditions

Aliquot the LKB1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the LKB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Chicken, Mouse, Rat

Image Legend

Confocal immunofluorescent analysis of LKB1 antibody with ZR-75-1 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .