AKT1 Antibody

AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. It and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P31749

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 115-144 from the human protein was used as the immunogen for this AKT1 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, WB, IF, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the AKT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This AKT1 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the AKT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.