PGK1 Antibody
Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
P00558
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 117-145 from the human protein was used as the immunogen for this PGK1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, IF, FACS, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the PGK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PGK1 antibody is available for research use only.
Storage Conditions
Aliquot the PGK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Rat
Location
Nuclear, cytoplasmic
Image Legend
IHC analysis of FFPE human kidney section using PGK1 antibody; Ab was diluted at 1:100.
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