AKT2 Antibody
AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:10-1:50, Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50
UniProt
P31751
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 452-481 from the human protein was used as the immunogen for this AKT2 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, IF, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the AKT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This AKT2 antibody is available for research use only.
Storage Conditions
Aliquot the AKT2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat
Image Legend
Fluorescent confocal image of C2C12 cell stained with AKT2 antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
Frequently Asked Questions
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