AKT2 Antibody
AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
P31751
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 93-123 from the human protein was used as the immunogen for this AKT2 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, IF, FACS, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the AKT2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This AKT2 antibody is available for research use only.
Storage Conditions
Aliquot the AKT2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of AKT2 antibody and 293 lysate transiently transfected with the human gene (2ug/lane) . Predicted molecular weight: ~56kDa.
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