Aurora-C Antibody

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

Q9UQB9

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 3-38 from the human protein was used as the immunogen for this Aurora-C antibody.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Aurora-C antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Aurora-C antibody is available for research use only.

Storage Conditions

Aliquot the Aurora-C antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Aurora-C antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescence staining of HeLa cells expressing GFP-Aurora-C performed at different cellular mitotic stages with the A) Aurora-C antibody, B) GFP fluorescence, C) DAPI nuclear staining, and D) anti-Aurora C merged to DAPI staining.