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CD28 Antibody

CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50

UniProt

P10747

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 182-208 from the human protein was used as the immunogen for this CD28 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CD28 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD28 antibody is available for research use only.

Storage Conditions

Aliquot the CD28 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CD28 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Rabbit

Image Legend

Confocal immunofluorescent analysis of CD28 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .

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